CST, 26390SF, Integrin beta4 (D8P6C) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Integrin beta4. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Integrin beta4 (D8P6C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #26390) is ready to ship. Product Usage Information This product is the carrier free version of product #14803. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14803 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Integrin beta4 (D8P6C) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total integrin β4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human integrin β4 protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). Integrin β4 pairs with integrin α6 on the cell surface membrane to form the integrin α6β4 heterodimer, an important laminin receptor that is essential for hemidesmosome formation and the support of stable adhesions between basal epithelial cells and the basement membrane (4,5). Integrin β4 is an important component in several growth factor induced signaling pathways that are involved in tumorigenesis and invasive cell growth (6,7). Alternate Names CD104; CD104 antigen; GP150; Integrin beta-4; integrin beta-4 subunit; integrin subunit beta 4; integrin, beta 4; ITB4; ITGB4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 210 Source/Isotype: Rabbit IgG