CST, 2639S, Fibrillarin (C13C3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Fibrillarin. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Fibrillarin (C13C3) Rabbit Monoclonal Antibody (CST #2639) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 20591 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Fibrillarin (C13C3) Rabbit Monoclonal Antibody detects endogenous levels of total fibrillarin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Thr298 of human fibrillarin. Background Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structural proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure, and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5). Alternate Names 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL; FIB; FIB1; fibrillarin; FLRN; Histone-glutamine methyltransferase; Nop1; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin; U6 snRNA 2'-O-methyltransferase fibrillarin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG