CST, 26533S, ANGPTL3 (F8N1Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ANGPTL3. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ANGPTL3 (F8N1Q) Rabbit Monoclonal Antibody (CST #26533) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ANGPTL3 (F8N1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ANGPTL3 protein. This antibody recognizes both the full-length protein (UniProt #Q9Y5C1) and the cleaved C-terminal fibrinogen-like domain (ANGPTL3-FLD). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ANGPTL3 protein. Background Angiopoietin-like 3 (ANGPTL3) is a secreted protein primarily expressed in liver and a regulator of fatty acid homeostasis (1). The angiopoietin-like family contains a signal peptide, an N-terminal coiled-coil domain (CCD), a linker peptide, and a C-terminal fibrinogen-like domain (FLD). Cleavage of the CCD and FLD occurs within the linker peptide, and is required for the activity of both domains (2). ANGPTL3-CCD, in concert with ANGPTL4/ANGPTL8, increases circulating triglyceride levels by inhibiting lipoprotein lipase, a hydrolase of LDL cholesterol (2,3). ANGPTL3-CCD also directly inhibits endothelial lipase, the enzyme responsible for HDL cholesterol hydrolysis (8,9). Thus, ANGPTL3-deficient mice have low levels of plasma HDL cholesterol, a risk factor for atherosclerosis (8,9). Unlike traditional angiopoietins, ANGPTL3-FLD does not bind the Tie2 receptor (4). ANGPTL3-FLD has been demonstrated to stimulate angiogenesis in endothelial cells via binding to integrin αVβ3, and to facilitate lipolysis in adipocytes via stimulation of the PDGFRβ-BRAF-ERK pathway (4,5). ANGPTL3 is currently a therapeutic target in patients with high-LDL dyslipidaemia, and can be inhibited with the use of monoclonal antibodies or antisense nucleotides (6,7). Alternate Names ANG-5; angiopoietin 5; angiopoietin like 3; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGL3; ANGPT5; ANGPTL3; ANGPTL3(17-221); ANGPTL3(17-224); ANL3; FHBL2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-38, 55-65 Source/Isotype: Rabbit IgG