CST, 2657S, Lck (L22B1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Lck. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Lck (L22B1) Mouse Monoclonal Antibody (CST #2657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Lck (L22B1) Mouse Monoclonal Antibody detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a GST-Lck fusion protein containing amino acid residues 1 to 234 of human Lck. Background The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Alternate Names IMD22; LCK; LCK proto-oncogene, Src family tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56-LCK; p56(LSTRA) protein-tyrosine kinase; p56lck; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Lck; YT16 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Mouse IgG1