CST, 26589S, CD4 (RM4-5) Rat Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying CD4 mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD4 (RM4-5) Rat Monoclonal Antibody (PE Conjugate) (CST #26589) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.25 μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:80 Flow Cytometry (Live): 1:80 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD4 (RM4-5) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD4 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1). Alternate Names Cd4; CD4 antigen; L3T; L3T4; Ly-; Ly-4; OTTMUSP00000027399; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4) Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2a kappa