CST, 2659S, Emerin (L12) Antibody

Polyclonal Antibody for studying Emerin. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Emerin (L12) Antibody (CST #2659) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Emerin (L12) Antibody detects endogenous levels of total Emerin protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu12 of human Emerin. Antibodies are purified by peptide affinity chromatography. Background Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that emerin is required for HIV-1 infectivity (9). Mutations in the gene encoding emerin ( ) are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10). Alternate Names EDMD; EMD; Emerin; LEM domain containing 5; LEMD5; STA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 SOURCE: Rabbit