CST, 26614S, SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SERPINB3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody (CST #26614) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67517 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody recognizes endogenous levels of total SERPINB3 protein. This antibody detects a 25 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu323 of human SERPINB3 protein. Background Serpin family B member 3 (SERPINB3), also known as squamous cell carcinoma antigen 1 (SCCA1), belongs to the serpin family and inhibits papain-like cysteine proteases (1). SERPINB3 is unique to the other serpins, as it lacks inhibitory activity against serine proteases but has inhibitory activity against papain-like cysteine proteases cathepsins K, L, and S (1,2). It may function as an inhibitor to modulate the host immune response against tumor cells and can act as a UV-induced apoptosis inhibitor by suppressing the activity of c-Jun NH2-terminal kinase 1 (JNK1) (1,3). SERPINB3 is expressed in normal squamous epithelium and in several organs, including tongue, esophageal, uterine, cervical, and vaginal epithelium. It is also expressed in Hassall's corpuscles of the thymus and some areas of the skin (4). SERPINB3 has been found to be overexpressed and implicated in several types of cancer, including hepatocellular carcinoma (HCC), lung, ovarian, and breast cancer (5-8). It is also overexpressed and implicated in hepatoblastoma, autoimmune disorders, lung fibrosis, cholesteatoma, and skin disease (9-13). Alternate Names HsT1196; Protein T4-A; SCC; SCCA; SCCA-1; SCCA-PD; SCCA1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin family B member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; SERPINB3; SPB3; Squamous cell carcinoma antigen 1; SSCA1; T4-A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG