CST, 26648S, SPARCL1 (F3V2I) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SPARCL1 mouse. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, SPARCL1 (F3V2I) Mouse Monoclonal Antibody (CST #26648) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity SPARCL1 (F3V2I) Mouse Monoclonal Antibody recognizes endogenous levels of total SPARCL1 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val136 of mouse SPARCL1 protein. Background SPARC-like protein 1 (SPARCL1) is a cysteine-rich protein secreted into the extracellular matrix that belongs to the SPARC protein family. SPARC proteins (secreted protein acidic and rich in cysteine) are secreted glycoproteins involved in tissue renewal and remodeling, and embryonic development (1). SPARC proteins are highly upregulated in melanoma and other tumor types, but their contributing functions are not yet understood (2). However, the role SPARC proteins play in regulation of extracellular matrix interactions is likely to contribute to melanoma development and metastasis. SPARCL1 is likely to play a similar role in extracellular matrix remodeling in the vasculature and the brain. For example, SPARCL1 is upregulated in vascular endothelial cells in response to injury and infection, both remodeling the extracellular matrix and promoting pro-inflammatory macrophage activation (3). SPARCL1 is also expressed in glial cells in the brain, specifically radial glial cells during brain development and in mature astrocytes where it may play a role in the formation of glutamatergic synapses (4,5). Alternate Names Ecm2; Extracellular matrix protein 2; hevin; mas; mast9; Matrix glycoprotein Sc1; Sc1; SPARC-like 1; SPARC-like 1 (mast9, hevin); SPARC-like protein 1; Sparcl1; SPRL1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Mouse IgG1 kappa