CST, 26703S, CD11 alpha (E5S9K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITGAL/CD11a. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD11 alpha (E5S9K) Rabbit Monoclonal Antibody (CST #26703) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 36769 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD11 alpha (E5S9K) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD11α protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val975 of human CD11α protein. Background CD11α, also known as integrin alpha-L (ITGAL), is the alpha chain of the lymphocyte function-associated antigen-1 (LFA-1). CD11α combines with the beta chain CD18 (ITGB2) to form LFA-1, an integrin expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands ICAM-1, 2, 3, and 4 (1), F11R/JAM-1 (2,3), and the secreted form of ubiquitin-like protein ISG15 (4). LFA-1 is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction and transmigration (2,3), cytotoxic T cell co-stimulation (5), cytotoxic NK cell mediated killing (6), leukocyte clearance (7), and antibody-dependent killing by granulocytes and monocytes (8,9). LFA-1 is required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (10). High expression of CD11α has been associated with antigen-specific and tumor-reactive T cells (11,12). Alternate Names alpha polypeptide); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11A; Integrin alpha-L; integrin gene promoter; integrin subunit alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; ITAL; ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA-1A; LFA1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG