CST, 26730S, HERPUD1 Antibody

Polyclonal Antibody for studying HERPUD1. Validated for Western Blotting. Highly specific and rigorously validated in-house, HERPUD1 Antibody (CST #26730) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HERPUD1 Antibody recognizes endogenous levels of total HERPUD1 protein. This antibody also cross-reacts with unidentified proteins of 30 kDa and 48 kDa. This antibody does not cross-react with HERPUD2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HERPUD1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The homocysteine-responsive, ER-resident ubiquitin-like domain member 1 protein (HERPUD1) is an ER membrane protein and putative component of the ER-membrane-associated protein degradation (ERAD) pathway (1). HERPUD1 is a transmembrane protein with both amino- and carboxy-termini exposed to the cytoplasm. The amino-terminal HERPUD1 region contains an ubiquitin-like (UBL) domain (1). Increased expression of HERPUD1 protein following ER stress and its association with ERAD component proteins suggests that HERPUD1 may play an integral role in ERAD pathway function (1). Research studies demonstrate that HERPUD1 associates with components of the ERAD machinery, such as the E3 ubiquitin ligase HRD1, which enhances HRD1-mediated ubiquitination and proteasomal degradation of potentially toxic ERAD substrates (2-4). The HERPUD1 protein may act as a molecular adaptor for the recruitment of ERAD machinery and the amino-terminal UBL domain may important for this function (3,4). The loss of HERPUD1 expression renders cells more susceptible to ER stress and apoptosis (5,6). Alternate Names HERP; HERP1; HERPUD1; homocysteine inducible ER protein with ubiquitin like domain 1; homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein; homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1; Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein; KIAA0025; Methyl methanesulfonate (MMF)-inducible fragment protein 1; MIF1; MMS-inducible; SUP Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit