CST, 2679T, Calnexin (C5C9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Calnexin. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Calnexin (C5C9) Rabbit Monoclonal Antibody (CST #2679) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53191 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Calnexin (C5C9) Rabbit Monoclonal Antibody detects endogenous levels of total calnexin protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro53 of human Calnexin. Background Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). Alternate Names Calnexin; CALX; CANX; CNX; epididymis secretory sperm binding protein; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG