CST, 26836L, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FN1. Validated for WB,IP,IHC,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (CST #26836) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44800 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total Fibronectin/FN1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein. Background Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6). Alternate Names Anastellin; CIG; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; epididymis secretory sperm binding protein; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF; Ugl-Y1; Ugl-Y2; Ugl-Y3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG