CST, 2684S, IKK beta Antibody

Polyclonal Antibody for studying IKK-beta. Validated for Western Blotting. Highly specific and rigorously validated in-house, IKK beta Antibody (CST #2684) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IKK beta Antibody detects endogenous levels of total IKKβ protein. This antibody does not cross-react with endogenous levels of IKKα or IKKγ. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human IKKβ. Antibodies are purified by protein A and peptide affinity chromatography. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). Alternate Names FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkappaB kinase; IKBKB; IKK-B; IKK-beta; IKK2; IKKB; IMD15; IMD15A; IMD15B; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa B kinase subunit beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 87 SOURCE: Rabbit