CST, 2689S, Phospho-IKK gamma (Ser376) Antibody

Polyclonal Antibody for studying IKK-gamma (Ser376) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IKK gamma (Ser376) Antibody (CST #2689) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IKK gamma (Ser376) Antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser376. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser376 of human IKKγ protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ primarily at Ser376 (14). In mouse, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimulation of IKKβ kinase activity (15). Alternate Names AMCBX1; EDAID1; FIP-3; FIP3; Fip3p; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKK-gamma; IKKAP1; IKKG; IMD33; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of nuclear factor kappa B kinase regulatory subunit gamma; inhibitor of nuclear factor kappa B kinase subunit gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; IP2; IPD2; NEMO; NF-kappa-B essential modifier; NF-kappa-B essential modulator; NFkappaB essential modulator; ZC2HC9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit