CST, 26902S, Nectin-2/CD112 (E4H9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying nectin 2. Validated for Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, Nectin-2/CD112 (E4H9V) Rabbit Monoclonal Antibody (CST #26902) is ready to ship. Product Usage Information Immunoprecipitation: 1:100 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity Nectin-2/CD112 (E4H9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Nectin-2/CD112 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Nectin-2/CD112 protein. Background Nectin-2, also known as CD112 and poliovirus receptor-related 2 (PVRL2), is a single-pass type I membrane glycoprotein ubiquitously expressed on various human tissues (1). It is a calcium independent cell adhesion molecule known to interact with several cell surface receptors, including DNAM-1 (CD226), LFA-1 (CD11a), Nectin-3 (CD113), TIGIT (VSTM3), and PVRIG (CD112R) (2-7). It is one of the major constituents of adherens junctions, and therefore plays a central role in a number of cellular processes, including adhesion, migration, and proliferation (2-8). Within the immune system, Nectin-2 modulates immune cell signaling. Upon interaction with DNAM-1 expressed on T and NK cells, Nectin-2 stimulates proliferation and cytokine production (4). Upon interaction with PVRIG, Nectin-2 inhibits proliferation (7). Thus, Nectin-2 can be either a co-stimulator or a co-inhibitor of immune cell function depending on competitive receptor interactions. Nectin-2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses (8,9). Alternate transcriptional splice variants, encoding different isoforms, have been characterized (10-12). Alternate Names CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; NECT2; nectin 2; Nectin cell adhesion molecule 2; Nectin-2; NECTIN2; poliovirus receptor related 2; poliovirus receptor-like 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); Poliovirus receptor-related protein 2; PRR2; PVRL2; PVRR2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-80 Source/Isotype: Rabbit IgG