CST, 2694T, Phospho-IKK alpha/beta (Ser176/180) Antibody II

Polyclonal Antibody for studying IKK-beta (Ser177/Ser181) phosphate/IKK-alpha (Ser176/Ser180) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IKK alpha/beta (Ser176/180) Antibody II (CST #2694) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IKK alpha/beta (Ser176/180) Antibody II detects endogenous levels of IKKα and IKKβ only when phosphorylated at Ser176 and Ser180 or Ser177 and Ser181, respectively. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a phosphopeptide corresponding to a region surrounding Ser177/181 of IKKβ. Antibodies are purified by protein A and peptide affinity chromatography. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). Alternate Names CHUK; component of inhibitor of nuclear factor kappa B kinase complex; conserved helix-loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; FLJ40509; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKBKB; IKK-A; IKK-a kinase; IKK-alpha; IKK-B; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; IMD15A; IMD15B; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa B kinase subunit beta; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; Transcription factor 16 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 IKK-alpha 87 IKK-beta SOURCE: Rabbit