CST, 2701S, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody

Polyclonal Antibody for studying ZAP70 (Tyr319) phosphate/Syk (Tyr352) phosphate. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (CST #2701) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography. Background The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9). Alternate Names 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; DKFZp313N1010; FLJ17670; FLJ17679; FLJ25043; FLJ37489; IMD48; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SRK; STCD; STD; SYK; Syk-related tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Zap-70, 72 Syk SOURCE: Rabbit