CST, 2703S, DYRK1B Antibody

Polyclonal Antibody for studying DYRK1B. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DYRK1B Antibody (CST #2703) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DYRK1B Antibody detects endogenous levels of total DYRK1B protein. This antibody detects the three major alternative splicing variants reported for DYRK1B. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human DYRK1B. Antibodies were purified by protein affintiy chromatography. Background The DYRK family includes several ual-specificity t rosine-phosphorylated and egulated inases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6). In contrast to the ubiquitous DYRK1A, DYRK1B exhibits relatively restricted expression with highest levels found in the testis and muscle (7,8). Three major DYRK1B splice variants demonstrate distinct expression patterns and functional properties (9). DYRK1B plays a critical role in myoblast differentiation by affecting cell motility, transcription, cell cycle progression and survival (10,11). In addition, DYRK1B contributes to the survival of certain cancer cells (7,12,13). Alternate Names AOMS3; dual specificity tyrosine phosphorylation regulated kinase 1B; dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B; DYR1B; DYRK1B; Minibrain-related kinase; MIRK; Mirk protein kinase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70-80 SOURCE: Rabbit