CST, 27072S, TWEAK Receptor/Fn14 (E6N2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TweakR. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TWEAK Receptor/Fn14 (E6N2I) Rabbit Monoclonal Antibody (CST #27072) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TWEAK Receptor/Fn14 (E6N2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TWEAK Receptor/Fn14 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly118 within the cytoplasmic region of human TWEAK Receptor/Fn14 protein. Background The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggested some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9). Alternate Names CD266; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; FN14; TNF receptor superfamily member 12A; TNFRSF12A; TNR12; Tumor necrosis factor receptor superfamily member 12A; Tweak-receptor; tweak-receptor; type I transmembrane protein Fn14; TweakR; type I transmembrane protein Fn14 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14-17 Source/Isotype: Rabbit IgG