CST, 2709S, Zap-70 (L1E5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ZAP70. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Zap-70 (L1E5) Mouse Monoclonal Antibody (CST #2709) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 59806 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Zap-70 (L1E5) Mouse Monoclonal Antibody detects endogenous levels of total Zap-70 protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Ser289 of human Zap-70. Background The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Alternate Names 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; FLJ17670; FLJ17679; IMD48; SRK; STCD; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Mouse IgG1