CST, 27140T, FKBP5 (F1G3I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FKBP5. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, FKBP5 (F1G3I) Rabbit Monoclonal Antibody (CST #27140) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity FKBP5 (F1G3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total FKBP5 protein. Non-specific cytoplasmic staining was observed in the epidermis of human skin by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly220 of human FKBP5 protein. Background FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) activity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8). Alternate Names 51 kDa FK506-binding protein; 51 kDa FK506-binding protein 5; 51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; AIG6; Androgen-regulated protein 6; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP prolyl isomerase 5; FKBP-5; FKBP-51; FKBP5; FKBP51; FKBP54; HSP90-binding immunophilin; MGC111006; p54; Peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase; PPIase FKBP5; Ptg-10; Rotamase; T-cell FK506-binding protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG