CST, 2716S, MO25 alpha/CAB39 (C49D8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MO25/CAB39. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MO25 alpha/CAB39 (C49D8) Rabbit Monoclonal Antibody (CST #2716) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MO25 alpha/CAB39 (C49D8) Rabbit Monoclonal Antibody detects endogenous levels of total MO25α/CAB39 and MO25β/CAB39L proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MO25α/CAB39 protein. Background AMPK (AMP-activated protein kinase) is a key cellular component that regulates energy homeostasis (1,2). When the AMP/ATP ratio is increased due to energy depletion, AMPK is phosphorylated at its α1 and α2 catalytic subunits and activated (3). Studies showed that the tumor suppressor LKB1 phosphorylates and activates AMPK in mammals (4,5). MO25α (mouse protein 25α), also called CAB39 (calcium binding protein 39), is a component of the LKB1 complex (5,6). MO25α/CAB39 does not directly interact with LKB1; instead, it is physically associated with STRADα (6). Together MO25α/CAB39 and STRADα help anchor LKB1 in the cytoplasm (6). MO25α/CAB39 stablizes the LKB1-STRADα complex and further increases the catalytic activity of LKB1 (6). Alternate Names CAB39; calcium binding protein 39; Calcium-binding protein 39; CGI-66; FLJ22682; MO25; MO25alpha; Protein Mo25 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Rabbit IgG