CST, 27182S, Rhodopsin (D4B9B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying rhodopsin. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Rhodopsin (D4B9B) Rabbit Monoclonal Antibody (CST #27182) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:1000 Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 59698 . Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Rhodopsin (D4B9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total rhodopsin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu194 of human rhodopsin protein. Background Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding (1). Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes (2). Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved (3). Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa (4,5), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness (6). Alternate Names CSNBAD1; MGC138309; MGC138311; OPN2; OPSD; opsin 2, rod pigment; Opsin-2; retinitis pigmentosa 4, autosomal dominant; RHO; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); RP4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG