CST, 27198T, beta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Beta Galactosidase. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, beta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody (CST #27198) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity beta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-galactosidase protein. This antibody cross-reacts with a 40 kDa protein of unknown identity in some cell extracts. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp39 of human β-galactosidase protein. Background β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene ( ) in the presence of a β-gal substrate to identify recombinant clones (1). In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells (2), SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence (3). Alternate Names Acid beta-galactosidase; Beta-galactosidase; BGAL; EBP; elastin binding protein; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; GLB1; Lactase; MPS4B Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG