CST, 27211S, Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFH phosphate motif. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit Monoclonal Antibody (CST #27211) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit Monoclonal Antibody recognizes endogenous levels of Neurofilament-H protein only phosphorylated at Ser residues surrounded by Ala or Val at the -2 position, Lys at the -1 position, Pro at the +1 position, and Val, Ala, or Glu at the +2 position. This antibody recognizes the VKsPA motif most sensitively. Non-specific labeling of thymus and testis is observed by immunofluorescence in fixed frozen mouse tissue. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-Ser Neurofilament-H motif (A/V)KsP(V/A/E) peptide. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 200 kDa neurofilament protein; CMT2CC; KIAA0845; NEFH; Neurofilament H; neurofilament heavy; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF-H; NFH Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 180-220 Source/Isotype: Rabbit IgG