CST, 2721S, Wnt3a (C64F2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Wnt3a. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Wnt3a (C64F2) Rabbit Monoclonal Antibody (CST #2721) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Wnt3a (C64F2) Rabbit Monoclonal Antibody detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a. Background The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5). Alternate Names MGC119418; MGC119419; MGC119420; Protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt family member 3A; WNT3A Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 42 Source/Isotype: Rabbit IgG