CST, 2724S, Bcl-w (31H4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bcl-W. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Bcl-w (31H4) Rabbit Monoclonal Antibody (CST #2724) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bcl-w (31H4) Rabbit Monoclonal Antibody detects endogenous levels of total Bcl-w protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding alanine 39 of Bcl-w. Background The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok; and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. The pro-survival protein Bcl-w was originally identified in a PCR-based strategy aimed at discovering novel Bcl-2 family members and was found to be expressed in cells of myeloid origin, as well as many other tissues (6,7). Most tissues from knockout mice were unaffected, but male mice did show defects in seminiferous tubule organization and spermatogenogenesis (8,9). Alternate Names Apoptosis regulator Bcl-W; B2CL2; Bcl-2-like protein 2; BCL-W; BCL2 like 2; Bcl2-L-2; BCL2-like 2; BCL2-like 2 protein; BCL2L2; BCLW; KIAA0271; PPP1R51; protein phosphatase 1, regulatory subunit 51 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG