CST, 27269T, ChAT (E4F9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ChAT. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ChAT (E4F9G) Rabbit Monoclonal Antibody (CST #27269) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ChAT (E4F9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total human, mouse, and rat ChAT protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ChAT protein. Background Choline O-acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from acetyl CoA and choline in the central and peripheral nervous system (1). In mammals, ACh is the primary neurotransmitter used at the neuromuscular junction, released by motor neurons to activate muscles. Neurons that release ACh are known as cholinergic neurons, which express high levels of ChAT. In addition to motor neurons, cholinergic neurons are present in the brain, particularly in the basal forebrain as well as other regions of the brain, including striatal interneurons. Reduction in ChAT, indicative of degeneration of cholinergic neurons, is correlated with cognitive decline in several neurodegenerative diseases, including Alzheimer's disease (2). Alternate Names acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O-acetyltransferase; CLAT; CMS1A; CMS1A2; CMS6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 71 Source/Isotype: Rabbit IgG