CST, 27281S, RNase L (D4B4J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RNAseL. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RNase L (D4B4J) Rabbit Monoclonal Antibody (CST #27281) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RNase L (D4B4J) Rabbit Monoclonal Antibody recognizes endogenous levels of total RNase L protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro717 of human RNase L protein. Background RNase L is an antiviral protein that is expressed in most mammalian cells (1). Latent RNase L in the cytoplasm is activated by the second messenger 2',5'-linked oligoadenylate (2-5A), which is produced by oligoadenylate synthase (OAS) after it binds viral double-stranded RNA (dsRNA) (2, 3). RNase L forms a crossed homodimer that is stabilized by kinase homology and ankyrin domains, which position two kinase extension nuclease domains for RNA recognition (4). RNase L then degrades both viral and cellular RNA (5). In mouse models, RNase L has been shown to produce small self-RNAs that act to amplify innate antiviral immunity through IFN-β induction (6). Research has also shown that RNase L forms a complex with Filamin A that acts as a barrier to restrict virus entry, and that RNase L can induce autophagy in response to viral infection (7, 8). Finally, research suggests RNase L may contribute to type I diabetes onset through immune response regulation (9). Alternate Names 2-5A-dependent ribonuclease; 2-5A-dependent RNase; 2',5'-oligoisoadenylate synthetase-dependent; DKFZp781D08126; interferon-induced 2-5A-dependent RNase; MGC104972; MGC133329; PRCA1; Ribonuclease 4; Ribonuclease L; ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RN5A; RNase L; RNASEL; RNS4 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG