CST, 27297S, p22phox Antibody

Polyclonal Antibody for studying CYBA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, p22phox Antibody (CST #27297) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity p22phox Antibody recognizes endogenous levels of total p22phox protein. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p22phox protein. Antibodies are purified by peptide affinity chromatography. Background The p22phox protein is a critical component of the membrane-bound NADPH Oxidase of phagocytes that generate superoxide anion and alter the redox states in cells. p22phox associates with the catalytic subunit gp91phox (NOX2), NOX1, NOX3, or NOX4 to form the active NADPH Oxidase. The enzymatic complex includes cytosolic subunits p40phox, p47phox, p67phox, and Rac2 that are recruited to the membrane-bound subunits, subsequently generating superoxide anion as the final product, serving as a microbial killing system (1,2). Multiple phosphorylation sites in each subunit of these oxidases potentiate its activation, including the phosphorylation of p22phox at Thr147 epitope facing the cytoplasmic space (3-5). NADPH Oxidase is associated with several diseases, including hyperglycemia, cancer, inflammation and vascular cases, as well as neurodegenerative diseases (6), including its activation by SOD1 mutants in Amyotrophic Lateral Sclerosis (7), and in Alzheimer's disease where NADPH Oxidase activation exacerbates the effects of fibrillary tangles and β-amyloid plaques, causing neuronal death (8,9). Alternate Names CGD4; CY24A; CYBA; cytochrome b light chain; cytochrome b-245 alpha chain; Cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; cytochrome b, alpha polypeptide; Cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; Cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit