CST, 2729S, Normal Rabbit IgG

Polyclonal Antibody for studying . Validated for Immunoprecipitation,Chromatin IP,CUT&Tag. Highly specific and rigorously validated in-house, Normal Rabbit IgG (CST #2729) is ready to ship. Product Usage Information Important! Dilute this control antibody to the same concentration (not dilution) as the specific test antibody in the chromatin immunoprecipitation. This is typically 1 μl (1 μg) to 5 μl (5 μg) of control antibody for one immunoprecipitation. Higher background signal may result if an excessive amount of rabbit IgG isotype control is used. Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Source / Purification The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography. Background Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Specification Concentration: 1 mg/ml SENSITIVITY: Endogenous Source/Isotype: Rabbit