CST, 27317SF, Calpain 2 Large Subunit (M-type) (E3M6E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Calpain-2 LS. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Calpain 2 Large Subunit (M-type) (E3M6E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #27317) is ready to ship. Product Usage Information This product is the carrier free version of product #70655. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 70655 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Calpain 2 Large Subunit (M-type) (E3M6E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total calpain 2 (large subunit) protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calpain 2 (large subunit) protein. Background Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). , calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin), and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1). Alternate Names Calcium-activated neutral proteinase 2; Calpain 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; Calpain-2; Calpain-2 catalytic subunit; Calpain-2 large subunit; calpain, large polypeptide L2; CAN2; CANP 2; CANP2; CANPL2; CANPml; CAPN2; FLJ39928; M-calpain; mCANP; Millimolar-calpain Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG