CST, 2735S, XRCC1 Antibody

Polyclonal Antibody for studying XRCC1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, XRCC1 Antibody (CST #2735) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity XRCC1 Antibody detects endogenous levels of total XRCC1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Arg300 of human XRCC1. Antibodies are purified by protein A and peptide affinity chromatography. Background The X-ray repair cross-complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8). Alternate Names DNA repair protein XRCC1; RCC; SCAR26; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross complementing 1; X-ray repair cross complementing protein 1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster; XRCC1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 SOURCE: Rabbit