CST, 27425SF, GATA-1 (D24E4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GATA1. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, GATA-1 (D24E4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #27425) is ready to ship. Product Usage Information This product is the carrier free version of product #4589. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4589 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GATA-1 (D24E4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of GATA-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala184 of human GATA-1. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in the corresponding gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9). Alternate Names ERYF1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA1; GF-1; GF1; globin transcription factor 1; NF-E1; NF-E1 DNA-binding protein; NFE1; nuclear factor, erythroid 1; transcription factor GATA1; XLANP; XLTDA; XLTT Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG