CST, 2744S, SPINK3 Antibody

Polyclonal Antibody for studying Spink3. Validated for Western Blotting. Highly specific and rigorously validated in-house, SPINK3 Antibody (CST #2744) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SPINK3 Antibody detects endogenous levels of total SPINK3 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the mouse SPINK3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The Kazal type 3 serine peptidase inhibitor (SPINK3) is a serine peptidase inhibitor expressed in the adult pancreas that protects acinar cells from trypsin-induced cell damage (1). Ingestion of milk by newborn SPINK3 knock out mice triggers autophagic cell death in pancreatic acinar cells and the complete disappearance of the pancreas (2). Mutations in the human SPINK3 homolog (known as SPINK1) are associated with chronic pancreatitis, presumably through decreased protection against trypsin-induced autophagic cell death (1,3). SPINK3 may be involved in the embryonic development of several other tissues (4). Alternate Names ISK1; ISK3; P12; prostatic secretory glycoprotein; serine peptidase inhibitor, Kazal type 1; serine peptidase inhibitor, Kazal type 3; Serine protease inhibitor Kazal-type 1; serine protease inhibitor Kazal-type 3; serine protease inhibitor, Kazal type 3; Spink1; Spink3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 10 SOURCE: Rabbit