CST, 27450T, HBEGF (E5L5T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HBEGF. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HBEGF (E5L5T) Rabbit Monoclonal Antibody (CST #27450) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HBEGF (E5L5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total proHBEGF or mature HBEGF protein. This antibody also detects a 58 kDa band of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human mature HBEGF protein. Background Heparin-binding EGF-like growth factor (HBEGF), also known as HB-EGF, belongs to the epidermal growth factor (EGF) family (1). The newly synthesized proHBEGF (about 27 kDa) has an N-terminal prodomain, a heparin-binding EGF domain, and a C-terminal transmembrane fragment (CTF). The heparin-binding region of HBEGF interacts with heparin residues in the extracellular matrix (ECM) and anchors the protein in the ECM. The proHBEGF is activated by the extracellular ADAM and MMP metalloproteases, shedding off its N-terminal prodomain and/or CTF, resulting in a 14-22 kDa product depending on different proteolytic processes. The mature HBEGF acts as a ligand for EGFR or ErbB4 receptors to activate downstream signaling pathways (2,3). The remaining membrane bound CTF is further internalized and binds to Bcl6 to regulate the related nuclear function (3). HBEGF has been shown to be involved in many physiological processes, such as placenta formation (4), heart development and function (5), and hematopoietic cell proliferation (6). The growth factor plays important roles in tissue inflammation, wound healing, and cancer development. Therefore, HBEGF is proposed as a therapeutic target in related diseases (7-10). Alternate Names Diphtheria toxin receptor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); DT-R; DTR; DTS; DTSF; HB-EGF; HBEGF; HEGFL; heparin binding EGF like growth factor; Heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; Proheparin-binding EGF-like growth factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18, 21, 27 Source/Isotype: Rabbit IgG