CST, 27469T, DGAT1 (F7A3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DGAT1. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DGAT1 (F7A3Z) Rabbit Monoclonal Antibody (CST #27469) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DGAT1 (F7A3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGAT1 protein. This antibody does not cross-react with DGAT2 protein. Faint nuclear background may be observed by immunofluorescence. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val31 of human DGAT1 protein. Background Diacylglycerol O-acyltransferase 1 (DGAT1) catalyzes triacylglycerol biosynthesis from diacylglycerol and fatty acyl-CoA (1). Studies show that is highly expressed in glioblastoma (GBM), stimulating the storage of excess fatty acids in triacylglycerols and lipid droplets to block GBM cancer cells from oxidative damage. DGAT1 inhibition in GBM cancer cells enhances reactive oxygen species (ROS) production and causes mitochondrial damage, leading to cancer cell death (2). In addition, Jumonji domain-containing 6 (JMJD6) is critical for clear cell renal cell carcinoma (ccRCC) development. JMJD6, along with RBM39, promotes expression. DGAT1 inhibition suppresses ccRCC tumorigenesis (3). Alternate Names ACAT related gene product 1; ACAT-related gene product 1; acyl coenzyme A:cholesterol acyltransferase related gene 1; Acyl-CoA retinol O-fatty-acyltransferase; acyl-CoA:diacylglycerol acyltransferase; AGRP1; ARAT; ARGP1; DGAT; DGAT1; Diacylglycerol O-acyltransferase 1; diacylglycerol O-acyltransferase homolog 1 (mouse); DIAR7; Diglyceride acyltransferase; Retinol O-fatty-acyltransferase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG