CST, 27482T, SAFB2 (F8F1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SAFB2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SAFB2 (F8F1A) Rabbit Monoclonal Antibody (CST #27482) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SAFB2 (F8F1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAFB2 protein. This antibody does not cross-react with SAFB1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala756 of human SAFB2 protein. Background Scaffold attachment factor B1 and B2 (SAFB1/B2) are homologs that bind to scaffold or matrix attachment region DNA elements (S/MAR DNA) (1,2). SAFB1 and SAFB2 bind to estrogen receptor to inhibit its activity, an action enhanced by tamoxifen treatment and opposed by histone deacetylase activity (2-4). SAFB1 and SAFB2 contain an RNA recognition motif and can interact with various hnRNP complexes and serine/arginine-rich splicing factors to influence pre-mRNA splicing (5-7). SAFB1 is also capable of helping in the DNA damage response by allowing yH2AX spreading (8). Knockdown of SAFB1 results in increased SAFB2 expression, and knockdown of SAFB2 results in increased SAFB1 expression (9). Despite their high homology, SAFB1 and SAFB2 have some non-redundant functions. SAFB1 knockout mice have a high degree of growth and fertility issues, while SAFB2 knockout mice have a normal phenotype but aberrations in Sertoli cells (10). SAFB proteins are expressed lowly in breast cancers with poor prognosis (11). Alternate Names KIAA0138; SAF-B2; SAFB2; Scaffold attachment factor B2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG