CST, 27502S, Mono-Methyl Arginine (R*GG) (D5A12) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, Mono-Methyl Arginine (R*GG) (D5A12) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #27502) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Mono-Methyl Arginine (R*GG) (D5A12) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of proteins only when mono-methylated at arginine. This antibody conjugate prefers Arg-Gly-Gly motifs. This antibody conjugate does not cross-react with di-methyl arginine or unmethylated arginine. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic mono-methyl arginine peptide library containing R*GG motifs. Background Arginine methylation is a prevalent PTM found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,NG-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-methylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, 6, and 8) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit