CST, 27543SF, Syk (D3Z1E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Syk. Validated for WB,IHC,IF,F,ELISA+. Highly specific and rigorously validated in-house, Syk (D3Z1E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #27543) is ready to ship. Product Usage Information This product is the carrier free version of product #13198. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13198. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Syk (D3Z1E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Syk protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Asn463 of human Syk protein. Background Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9). Alternate Names DKFZp313N1010; FLJ25043; FLJ37489; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SYK; Tyrosine-protein kinase SYK Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG