CST, 27545S, IL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying IL6. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #27545) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human IL-6 protein. Background Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6 family members, such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Rα, 80 kDa) and two beta chains (IL-6Rβ or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways: JAK/STAT, PI-3 kinase, and MAPK signaling pathways (2,3). Alternate Names B cell stimulatory factor-2; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; BSF2; CDF; CTL differentiation factor; HGF; HSF; Hybridoma growth factor; IFN-beta-2; IFNB2; IL-6; IL6; Interferon beta-2; interleukin 6; interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin-6 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG