CST, 2761S, Numb (C44B4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Numb. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Numb (C44B4) Rabbit Monoclonal Antibody (CST #2761) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Numb (C44B4) Rabbit Monoclonal Antibody detects endogenous levels of Numb protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein. Background Numb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9). Alternate Names c14_5527; C14orf41; h-Numb; NUMB; NUMB endocytic adaptor protein; numb homolog; numb homolog (Drosophila); Protein numb homolog; Protein S171; S171 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72, 74 Source/Isotype: Rabbit