CST, 27629T, KIR2DL2/KIR2DL3 (F1D9K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KIR2DL2. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, KIR2DL2/KIR2DL3 (F1D9K) Rabbit Monoclonal Antibody (CST #27629) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity KIR2DL2/KIR2DL3 (F1D9K) Rabbit Monoclonal Antibody recognizes endogenous levels of total KIR2DL2 and KIR2DL3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human KIR2DL2 protein. Background Killer cell immunoglobulin-like receptors (KIRs) are type 1 transmembrane glycoproteins expressed by natural killer (NK) cells and subsets of CD4, CD8, and γδ T cells (1-5). Analogous to the diversity of their human leukocyte antigen class I (HLA class I) ligands, the KIR genes are polymorphic and the content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (6,7). The KIR proteins are characterized by the number of extracellular immunoglobulin-superfamily domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain (8-10). KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) (10), while KIR proteins with the short cytoplasmic domain lack an ITIM and instead transduce activating signals (11,12). KIR proteins play an important role in the regulation of the immune response. Combinations of KIR and HLA class I variants influence susceptibility to autoimmunity and infectious disease, as well as outcomes of haematopoietic stem cell transplantation (12-14). KIR2DL2 and KIR2DL3, also referred to as CD158b1 and CD158b2, respectively, regulate NK cells by interacting with the human leukocyte antigen-C1 (HLA-C1) group of molecules (15). Upon receptor ligand interaction, KIR2DL2 and KIR2DL3 inhibit the activity of NK cells thus preventing target cell lysis (16-18). Alternate Names CD158 antigen-like family member B1; CD158b; CD158B1; KI2L2; killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 2; Killer cell immunoglobulin-like receptor 2DL2; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 2; KIR2DL2; MHC class I NK cell receptor; Natural killer-associated transcript 6; NKAT-6; NKAT6; p58 natural killer cell receptor clone CL-43; p58 NK receptor CL-43; p58.2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-70 Source/Isotype: Rabbit IgG