CST, 27643S, Cleaved Caspase-6 (Asp162) (F6J4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CASP6 (Asp162, C-term) cleaved mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-6 (Asp162) (F6J4I) Rabbit Monoclonal Antibody (CST #27643) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cleaved Caspase-6 (Asp162) (F6J4I) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-6 protein only when cleaved at Asp162. This site refers to the cleavage site in rodent producing the p18 subunit. The homologous cleavage site in human is at Asp179. Weak reactivity may be observed for full-length caspase-6 in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp162 of mouse caspase-6 protein. Background Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane-associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death. Alternate Names Apoptotic protease Mch-2; CASP-6; Casp6; caspase 6; Caspase-6; Caspase-6 subunit p11; Caspase-6 subunit p18; mCAS; mCASP-6; Mch2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG