CST, 2764S, Bcl-xL (54H6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCL-xL. Validated for WB,WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Bcl-xL (54H6) Rabbit Monoclonal Antibody (CST #2764) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:600 - 1:2400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 61060 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Bcl-xL (54H6) Rabbit Monoclonal Antibody detects endogenous levels of total Bcl-xL protein. This antibody does not cross-react with other Bcl-2 family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. Background Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5). Alternate Names Apoptosis regulator Bcl-X; B2CL1; Bcl-2-like protein 1; Bcl-X; bcl-xL; BCL-XL/S; bcl-xS; BCL2 like 1; Bcl2-L-1; BCL2-like 1; BCL2L; BCL2L1; BCLX; BCLXL; BCLXS; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG