CST, 2765S, Ribosomal Protein L13a Antibody

Polyclonal Antibody for studying RPL13A. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ribosomal Protein L13a Antibody (CST #2765) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ribosomal Protein L13a Antibody detects endogenous levels of total ribosomal protein L13a. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val185 of human ribosomal protein L13a. Antibodies are purified by peptide affinity chromatography. Background Ribosomal protein L13a (RPL13a, 60S ribosomal protein L13a) is a member of the L13 ribosomal protein family and a structural component of the 60S ribosomal subunit (1). RPL13a appears to play an important role in transcript-specific translational silencing. Interferon-γ induces the phosphorylation of RPL13a and triggers the release of this protein from the 60S ribosomal subunit (2). Free RPL13a protein binds to the GAIT (interferon-γ-activated inhibitor of translation) complex at the 3'-UTR of ceruloplasmin (Cp) mRNA to repress Cp expression (2). RPL13a bound to the GAIT complex interacts with eIF4G, which prevents the recruitment of 43S ribosomal subunit and results in transcript-specific translation suppression (3). Alternate Names 23 kD highly basic protein; 23 kDa highly basic protein; 60S ribosomal protein L13a; epididymis secretory sperm binding protein; L13A; Large ribosomal subunit protein uL13; ribosomal protein L13a; RL13A; RPL13A; tissue specific transplantation antigen 1; TSTA1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit