CST, 27693S, Recoverin (F2O5Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RCV1 mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Recoverin (F2O5Z) Rabbit Monoclonal Antibody (CST #27693) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Recoverin (F2O5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total recoverin protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly175 of mouse recoverin protein. Background Recoverin (Rcv), a small 23-kDa protein, is a member of the neuronal calcium sensor (NCS) family (1,2). It senses calcium levels in neurons and is primarily located in the outer segments of photoreceptors within the vertebrate retina, where it participates in light adaptation via calcium-dependent inhibition of the enzyme rhodopsin kinase (RK) (1). Rcv, like other family members, contains four EF-hand motifs and a myristoyl chain on the N-terminus. Only two of the EF-hands (EF2 and EF3) bind Ca across species (1). In darkness, Ca -bound Rcv inhibits G protein-coupled receptors (GPCRs) and thereby prevents visual pigment phosphorylation. Under bright light conditions, Ca dissociates from Rcv and the protein translocates to the inner segment. By this, Rcv prolongs phototransduction and enhances visual sensitivity (3). Few reports have described patients with anti-recoverin antibody-positive encephalitis (4). Alternate Names 23 kDa photoreceptor cell-specific protein; cancer associated retinopathy protein; Cancer-associated retinopathy protein; CAR; guanylate cyclase activator; MGC129468; OTTMUSP00000006130; Protein CAR; Rcv1; Rcvrn; RECO; Recoverin; S-mo; S-modulin Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG