CST, 2774S, Bax Antibody

Polyclonal Antibody for studying Bax. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Bax Antibody (CST #2774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Bax Antibody detects endogenous levels of total Bax protein. Cross-reactivity was not detected with other family members at physiological conditions. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lysine 58 of human Bax. Antibodies were purified by protein A and peptide affinity chromatography. Background Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4). Alternate Names Apoptosis regulator BAX; BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bcl-2-like protein 4; BCL2 associated X protein; BCL2 associated X, apoptosis regulator; BCL2-associated X protein; BCL2-associated X protein omega; Bcl2-L-4; BCL2L4 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 20 SOURCE: Rabbit