CST, 27789S, Gab2 (F6A4U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Gab2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Gab2 (F6A4U) Rabbit Monoclonal Antibody (CST #27789) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Gab2 (F6A4U) Rabbit Monoclonal Antibody recognizes endogenous levels of total Gab2 protein. This antibody does not cross-react with either Gab1 or Gab3 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly501 of human Gab2 protein. Background GRB2-associated-binding protein 2 (Gab2) belongs to the Grb-associated binder (Gab) family of signaling scaffolds, which are characterized by a highly conserved N-terminal PH domain for membrane recruitment, multiple proline-rich motifs for the recruitment of SH3 domain-containing proteins, such as GRB2, and multiple tyrosine residues capable of associating with SH2 domain-containing proteins, such as p85-PI3K, CrkL, and SHP-2, in a phosphorylation-dependent manner (1). Research studies have shown that Gab2 is a widely expressed protein that participates in orchestrating signals downstream of many transmembrane receptors for growth factors and cytokines (2). Indeed, Gab2 was initially identified as a tyrosine-phosphorylated protein associated with SHP-2 and the p85 subunit of PI3K in response to engagement of the IL-3 and M-CSF receptors (3,4). Subsequent studies also identified Gab2 as a participant in signaling cascades triggered by the T cell antigen receptor (5,6). It is now well established that Gab2 is a critical component of signaling cascades triggered by numerous oncogenic tyrosine kinases. In the context of human disease, Gab2 is overexpressed in breast cancer and ovarian cancer, which facilitates tumor cell proliferation and survival (7,8). Alternate Names GAB2; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated binding protein 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; pp100 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG