CST, 2779T, SOCS2 Antibody

Polyclonal Antibody for studying SOCS2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SOCS2 Antibody (CST #2779) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SOCS2 Antibody detects endogenous levels of SOCS2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human SOCS2. Antibodies are purified by peptide affinity chromatography. Background The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. Activity of SOCS2 has been predominantly linked to growth hormone (GH) and insulin-like growth factor 1 (IGF-1) signaling but may also contribute to several biological processes including metabolism, bone formation, neuronal development, cancer, infection and other cytokine-dependent pathways (5). SOCS2 is widely expressed in adult and fetal tissues and is induced upon cytokine treatment (5,6). A number of studies suggest that SOCS2 can have either a positive or negative effect on GH/cytokine signaling (7-10). Mice deficient in SOCS2 grow signficantly larger than normal littermates (8). SOCS2 binds to tyrosine-phosphorylated GH and IGF-1 receptors via its SH2 domain, suppressing their signaling (6,9). In addition, the SOCS box of SOCS2 binds to Elongin B and C leading to activity as a ubiquitin ligase, promoting the degradation of the receptors as well as other SOCS family members (11-13). Alternate Names CIS-2; CIS2; Cish2; Cytokine-inducible SH2 protein 2; SOCS-2; SOCS2; SSI-2; SSI2; STAT induced STAT inhibitor-2; STAT-induced STAT inhibitor 2; STATI2; Suppressor of cytokine signaling 2; suppressor of cytokine signaling-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit